FlowJo (macOS) keyboard shortcuts ‒ DefKey

0	⌘ Cmd + ↑ Shift + S	Save As
0	⌘ Cmd + S	Save
0	⌘ Cmd + Z	Undo
0	or ⌘ Cmd + Z
0	or ⌘ Cmd + Z
0	⌘ Cmd + F	Find
0	⌘ Cmd + A	Select All
0	or ⌘ Cmd + A
0	or ⌘ Cmd + A
0	⌘ Cmd + R	Rename
0	⌘ Cmd + R	Right
0	⌘ Cmd + L	Left
0	⌘ Cmd + P	Print
0	or ⌘ Cmd + P
0	or ⌘ Cmd + P
0	⌘ Cmd + V	Paste
0	or ⌘ Cmd + V
0	⌘ Cmd + F	Bring Forward
0	⌘ Cmd + I	Properties
0	⌘ Cmd + B	Send Backward
0	⌘ Cmd + X	Cut
0	or ⌘ Cmd + X
0	⌘ Cmd + W	Close Window
0	⌘ Cmd + G	Group
0	⌘ Cmd + U	Expand All
0	⌘ Cmd + U	Ungroup
0	⌘ Cmd + ,	Preferences
0	⌘ Cmd + ↑ Shift + F	Bring to Front
0	⌘ Cmd + Q	Exit
0	⌘ Cmd + Y	Preferences
0	⌘ Cmd + ↑ Shift + B	Send to Back
0	⌘ Cmd + ↑ Shift + U	Collapse All
0	⌘ Cmd + Del	Clear
0	or ⌘ Cmd + Del
0	or ⌘ Cmd + Del
0	Ctrl + G	Create Group
0	⌘ Cmd + S	Save Image As
0	⌘ Cmd + S	Save Graphic as
0	⌘ Cmd + F	To File
0	⌘ Cmd + F	Copy Histogram Function
0	⌘ Cmd + I	Inspect
0	⌘ Cmd + I	Copy Image with Transparency
0	⌘ Cmd + B	Add Statistic
0	⌘ Cmd + D	For Display
0	⌘ Cmd + B	Add Statistic
0	⌘ Cmd + D	Duplicate View
0	⌘ Cmd + N	New Workspace
0	⌘ Cmd + N	Add A New Layout
0	⌘ Cmd + N	New Table
0	⌘ Cmd + W	Close Workspace
0	⌘ Cmd + E	Export / Concantenate
0	⌘ Cmd + K	Vertical Spacing
0	⌘ Cmd + K	Batch to Clipboard
0	⌘ Cmd + E	Edit Column
0	⌘ Cmd + K	Next X
0	⌘ Cmd + G	New Group
0	⌘ Cmd + G	Manually Enter Gate
0	⌘ Cmd + O	Open Original Graph
0	⌘ Cmd + O	Open Child Population
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0	⌘ Cmd + ⤶ Enter	Open Graph Window
0	⌘ Cmd + L	Open Layout Editor
0	⌘ Cmd + L	Batch to Layout
0	⌘ Cmd + L	Copy to Layout Editor
0	⌘ Cmd + T	Open Table Editor
0	⌘ Cmd + T	Tops
0	⌘ Cmd + T	Transpose Axes
0	⌘ Cmd + -	Delete Layout
0	⌘ Cmd + -	Remove Table
0	⌘ Cmd + U	Up to Parent Population
0	⌘ Cmd + P	Batch to Printer
0	⌘ Cmd + ,	Create Combination Gates
0	⌘ Cmd + ↑ Shift + G	Copy Gate to Group
0	⌘ Cmd + ↑ Shift + C	Show/Hide Comp Nodes
0	⌘ Cmd + ↑ Shift + F	FCS Scan
0	⌘ Cmd + M	Compensation
0	⌘ Cmd + M	Make Movie
0	⌘ Cmd + ]	Customize Ribbons
0	⌘ Cmd + ↑ Shift + A	Make And Gate
0	⌘ Cmd + 3	Show in 3D
0	or ⌘ Cmd + 3
0	⌘ Cmd + 0	View Workspace
0	⌘ Cmd + 0	Show Workspace
0	⌘ Cmd + +	Figure Presets (Apply)
0	⌘ Cmd + ↑ Shift + M	Matrix Editor
0	⌘ Cmd + ↑ Shift + E	Select Equivalent Nodes
0	⌘ Cmd + ↑ Shift + D	Derived Parameters
0	⌘ Cmd + Y	Next Y
0	⌘ Cmd + ↑ Shift + N	Make Not Gate
0	⌘ Cmd + ↑ Shift + N	Add Column
0	⌘ Cmd + ↑ Shift + V	Check Samples Quality
0	⌘ Cmd + ↑ Shift + P	Panel Wizard
0	⌘ Cmd + ↑ Shift + I	Add Keyword
0	⌘ Cmd + ↑ Shift + O	Make Or Gate
0	⌘ Cmd + H	Horizontal Center
0	⌘ Cmd + J	Create Group from Keyword Value
0	⌘ Cmd + J	Horizontal Spacing
0	⌘ Cmd + J	Previous X
0	⌘ Cmd + ↑ Shift + K	Edit Columns
0	⌘ Cmd + ↑ Shift + B	Hide/Show Statistics”)
0	⌘ Cmd + 6	Create Keyword Value Series
0	⌘ Cmd + \	Bottoms
0	⌘ Cmd + ↑ Shift + W	Close All Graph Windows
0	⌘ Cmd + 9	Plate Editor
0	⌘ Cmd + ;	Add Samples
0	⌘ Cmd + =	Make Identical Gates
0	Command + Page Down	Vertical Center
0	Command + Page Down	Previous Sample
0	Command + Page Up	Next Sample
0	⌘ Cmd + F10	Previous Y
0	⌘ Cmd + Num /	Open Workspace
0	⌘ Cmd + =S	Duplicate Layout
0	⌘ Cmd + =S	Duplicate Table

General
FlowJo Tab
File tab
Edit Tab
Workspace Tab
Tools Tab
Settings Tab

Layout Tab
File Tab
Edit Tab
Object Tab
Arrange Tab

Tables Tab
Edit Menu
Columns Menu

General Shortcuts
Hold down Alt + Select the Axis Parameter in the Graph Window: Show the Uncompensated parameters in the Parameters drop down list
File Menu
Edit Menu
Graph Menu
Go Menu
Source: Flowjo documentation

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