FlowJo (macOS) keyboard shortcuts ‒ DefKey

General
0	⌘ Cmd + W	Close Workspace
0	⌘ Cmd + Q	Exit
0	⌘ Cmd + Z	Undo
0	⌘ Cmd + ]	Customize Ribbons
FlowJo Tab
0	⌘ Cmd + N	New Workspace
0	⌘ Cmd + ;	Add Samples
0	⌘ Cmd + G	New Group
0	⌘ Cmd + L	Open Layout Editor
0	⌘ Cmd + T	Open Table Editor
0	⌘ Cmd + Y	Preferences
0	⌘ Cmd + ↑ Shift + P	Panel Wizard
File tab
0	⌘ Cmd + Num /	Open Workspace
0	⌘ Cmd + P	Print
0	⌘ Cmd + S	Save
0	⌘ Cmd + E	Export / Concantenate
0	⌘ Cmd + ↑ Shift + S	Save As
0	⌘ Cmd + F	Find
0	⌘ Cmd + ↑ Shift + F	FCS Scan
Edit Tab
0	⌘ Cmd + X	Cut
0	⌘ Cmd + V	Paste
0	⌘ Cmd + Del	Clear
0	⌘ Cmd + A	Select All
0	⌘ Cmd + ↑ Shift + E	Select Equivalent Nodes
0	⌘ Cmd + ↑ Shift + U	Collapse All
0	⌘ Cmd + U	Expand All
Workspace Tab
0	Ctrl + G	Create Group
0	⌘ Cmd + ↑ Shift + G	Copy Gate to Group
0	⌘ Cmd + ⤶ Enter	Open Graph Window
0	⌘ Cmd + I	Inspect
0	⌘ Cmd + R	Rename
0	⌘ Cmd + B	Add Statistic
0	⌘ Cmd + ↑ Shift + I	Add Keyword
0	⌘ Cmd + J	Create Group from Keyword Value
0	⌘ Cmd + 6	Create Keyword Value Series
Tools Tab
0	⌘ Cmd + M	Compensation
0	⌘ Cmd + ↑ Shift + M	Matrix Editor
0	⌘ Cmd + ↑ Shift + V	Check Samples Quality
0	⌘ Cmd + ↑ Shift + D	Derived Parameters
0	⌘ Cmd + ,	Create Combination Gates
0	⌘ Cmd + ↑ Shift + O	Make Or Gate
0	⌘ Cmd + ↑ Shift + A	Make And Gate
0	⌘ Cmd + ↑ Shift + N	Make Not Gate
0	⌘ Cmd + =	Make Identical Gates
0	⌘ Cmd + 9	Plate Editor
0	⌘ Cmd + 3	Show in 3D
Settings Tab
0	⌘ Cmd + ↑ Shift + K	Edit Columns
0	⌘ Cmd + ↑ Shift + B	Hide/Show Statistics”)
0	⌘ Cmd + ↑ Shift + C	Show/Hide Comp Nodes

Layout Tab
0	⌘ Cmd + N	Add A New Layout
0	⌘ Cmd + =S	Duplicate Layout
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0	⌘ Cmd + -	Delete Layout
File Tab
0	⌘ Cmd + P	Print
0	⌘ Cmd + S	Save Image As
0	⌘ Cmd + 0	View Workspace
0	⌘ Cmd + O	Open Original Graph
0	⌘ Cmd + I	Properties
Edit Tab
0	⌘ Cmd + Z	Undo
0	⌘ Cmd + X	Cut
0	⌘ Cmd + V	Paste
0	⌘ Cmd + A	Select All
Object Tab
0	⌘ Cmd + +	Figure Presets (Apply)
Arrange Tab
0	⌘ Cmd + F	Bring Forward
0	⌘ Cmd + ↑ Shift + F	Bring to Front
0	⌘ Cmd + B	Send Backward
0	⌘ Cmd + ↑ Shift + B	Send to Back
0	⌘ Cmd + G	Group
0	⌘ Cmd + U	Ungroup
0	⌘ Cmd + T	Tops
0	⌘ Cmd + \	Bottoms
0	Command + Page Down	Vertical Center
0	⌘ Cmd + K	Vertical Spacing
0	⌘ Cmd + L	Left
0	⌘ Cmd + R	Right
0	⌘ Cmd + H	Horizontal Center
0	⌘ Cmd + J	Horizontal Spacing

Tables Tab
0	⌘ Cmd + N	New Table
0	⌘ Cmd + =S	Duplicate Table
0	⌘ Cmd + -	Remove Table
0	⌘ Cmd + D	For Display
0	⌘ Cmd + L	Batch to Layout
0	⌘ Cmd + K	Batch to Clipboard
0	⌘ Cmd + P	Batch to Printer
0	⌘ Cmd + F	To File
Edit Menu
0	⌘ Cmd + A	Select All
0	⌘ Cmd + Del	Clear
Columns Menu
0	⌘ Cmd + ↑ Shift + N	Add Column
0	⌘ Cmd + E	Edit Column

General Shortcuts
Hold down Alt + Select the Axis Parameter in the Graph Window: Show the Uncompensated parameters in the Parameters drop down list
File Menu
0	⌘ Cmd + W	Close Window
0	⌘ Cmd + ↑ Shift + W	Close All Graph Windows
0	⌘ Cmd + S	Save Graphic as
0	⌘ Cmd + P	Print
Edit Menu
0	⌘ Cmd + Z	Undo
0	⌘ Cmd + Del	Clear
0	⌘ Cmd + I	Copy Image with Transparency
0	⌘ Cmd + F	Copy Histogram Function
0	⌘ Cmd + L	Copy to Layout Editor
0	⌘ Cmd + B	Add Statistic
0	⌘ Cmd + ,	Preferences
Graph Menu
0	⌘ Cmd + D	Duplicate View
0	⌘ Cmd + T	Transpose Axes
0	⌘ Cmd + G	Manually Enter Gate
0	⌘ Cmd + M	Make Movie
0	⌘ Cmd + 3	Show in 3D
Go Menu
0	⌘ Cmd + 0	Show Workspace
0	⌘ Cmd + U	Up to Parent Population
0	⌘ Cmd + O	Open Child Population
0	Command + Page Down	Previous Sample
0	Command + Page Up	Next Sample
0	⌘ Cmd + J	Previous X
0	⌘ Cmd + K	Next X
0	⌘ Cmd + F10	Previous Y
0	⌘ Cmd + Y	Next Y
Source: Flowjo documentation

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